Hauv cov chaw soj ntsuam niaj hnub, kev nyab xeeb ntawm cov neeg ua haujlwm thiab ib puag ncig ib puag ncig yog qhov tseem ceeb tshaj plaws. Txhawm rau tiv thaiv kev kis tus kab mob muaj kuab thiab tshuaj lom neeg, ob hom khoom kaw kev nyab xeeb yog siv ntau:Biological txee (BSCS)thiabFums hoodsCov. Txawm hais tias lawv zoo sib xws nyob rau hauv cov tsos thiab qauv theem, lawv txawv nyob rau hauv kev ua haujlwm, npaj siv, thiab cov hom phiaj tiv thaiv.
Dab tsi yog aKev Nyab Xeeb Kev Nyab Xeeb CABSPET (BSC)?
Kev Siv Hluav Taws Xob Hluav Taws Xobyog hom khoom kuaj sim tsim los muab peb npaug kev tiv thaiv rau cov neeg ua haujlwm, kuaj sim, thiab ib puag ncigCov. Nws yog dav siv hauv kev siv hluav taws xob lom zem, tshwj xeeb tshaj yog thaum tuav tau cov kab mob uas txaus ntshai.
Hom BSC:
- Chav kawm I biosafety txee (BSC):Tiv thaiv tus neeg teb xov tooj thiab ib puag ncig, tab sis tsis yog tus qauv. Haum rau qes-} kev ua haujlwm pheej hmoo.
- Chav Kawm 2 Biosafety txee (BSC):Cov hom siv feem ntau siv, muab kev tiv thaiv rau cov neeg ua haujlwm, cov qauv, thiab ib puag ncig. Subtypes suav nrog:
- Version A1 / A2:Ib feem ntawm huab cua yog recirculated thiab ib feem ntawm huab cua yog sab sab.
- Hom B1 / B2:Ntau txoj kev tso tawm. Hom B2 yog 100% tso tawm thiab haum rau kev ua haujlwm nrog cov tshuaj vapors thiab pathogens.
- Chav Kawm Ntawv III BSC:Ib lub hnab looj tes uas tau muab tso rau hauv pob tes ...}} Style txee siv rau high- kev pheej hmoo lossis tsis paub pathogens (xws li hauv bsl-4 sim).
Cov Kev Siv Cov Ntaub Ntawv Feem Ntau:
- Kev coj noj coj ua mov ntawm tes
- Kov cov kab mob pathogenic microorganisms (xws li cov kab mob thiab kab mob)
- Molecular Biology Operations (piv txwv li RNA rho tawm, pcr kev npaj khoom)
- Aseptic Workflow
Dab tsi yog aFume hood?
Fums cov tais yog feem ntau siv los tiv thaiv cov neeg ua haujlwm ntsuas kev tsim txom, roj av lossis pilcorulates. Nws ua haujlwm los ntawm kev nqus cov pa mus rau hauv cov fume hood thiab tom qab ntawd sab sab nws tawm ntawm chav kuaj.
Hom fume hoods:
- Duced Dume Hood:Txos ua kom cua ncaj qha mus rau sab nraud ntawm lub tsev los ntawm kev sib dhos. Feem ntau dav siv.
- Ducel (recirculating) fume hoods:Siv kev lim dej sab hauv kom huv cov huab cua thiab tom qab ntawd recircating nws rov qab rau hauv chav kuaj. Pab tau thaum ducting tsis tau.
- Dedicated fums hoods:
- Acid -} Cov npog npog(ua los ntawm corrosion - Cov ntaub ntawv tiv taus)
- Perachloric acid hood(nrog dej ntws tawm)
- Tooj hlwb heev(nrog txhuas kev thaiv)
Cov Kev Siv Cov Ntaub Ntawv Feem Ntau:
- Npaj thiab lub kaum ntawm cov tshuaj lom neeg
- Hnyav evaporation sim
- Tuav cov hmoov txhuas los yog roj av
- Me - scale nplai organic synthesis
Qhov sib txawv tseem ceeb ntawm BSC thiab fume hood
| yam zoo | Kev Nyab Xeeb Kev Nyab Xeeb Kev Nyab Xeeb Txiaj Ntsig (BSC) | Fums hoods |
|---|---|---|
| Povhwm | Cov neeg ua haujlwm, kuaj thiab ib puag ncig | Cov neeg ua haujlwm nkaus xwb |
| Huab Cua Txaus | Hepa pom, recirculation lossis pa ncho | Ncaj qha tso rau sab nraud |
| Siv cov ntaub ntawv | Kev ua haujlwm ntawm roj ntsha tsis huv, pathogens | Tshuaj lom neeg phom sij, lom zem |
| kaw | Siab - tiv thaiv kev sib kis hluav taws xob biological | Nruab Nrab - Tiv Thaiv Cov Tshuaj Raug Tshuaj |
Thaum twg siv BSC hloov ntawm lub ntsej muag fum
✅ Siv BSC hauv cov xwm txheej hauv qab no:
- Kawm txog cov kab mob pathogenic microorganisms (kab mob, kab mob, fungi)
- Ua yeeb yam ntawm cov kab ke lossis cov txheej txheem aseptic
- Tiv thaiv cov qauv kev paug los ntawm qhov teeb meem lossis aerosols
- Tuav ntawm cov tsiaj lossis tib neeg cov hnoos qeev
✅ Siv ib lub ntsej muag zoo nyob hauv cov xwm txheej hauv qab no:
- Tuav cov tshuaj lom, khaus lossis cov tshuaj tsw qab
- Cov tshuaj tiv thaiv uas tso tawm cov pa roj (xws li ammonia thiab nitric acid vapor)
- Raug rau cov hmoov txhuv nplej siab lossis cov khoom muaj kev phom sij (piv txwv li nanomaterials)
- Khaws cia lossis faib cov kuab tshuaj uas muaj zog lossis cov kua qaub
Qhov yuam kev thiab cov lus qhia kev nyab xeeb
❌common:
- Tso cov khoom loj hauv cov txee, thaiv kev siv cua
- Siv cov kuab tshuaj hauv chav nyob hauv chav kawm 2 roj ntsha txee (BSC) (pheej hmoo ntawm hluav taws lossis tawg)
- Nquag qhib thiab kaw ntawm Windows lossis taug kev ncig yuav cuam tshuam rau kev ruaj ntseg ntawm airflow
❌commonFUME Hood yuam kev:
- Overloading ntawm thaj chaw ua haujlwm, thaiv ntawm cov huab cua pem hauv ntej
- Tsis quav ntsej cov lus pom zoo lossis ceeb toom airflow
- Muab koj lub taub hau rau hauv lub hood thaum lub sijhawm ua haujlwm
✅ Cov Lus Qhia Kev Nyab Xeeb:
- Nquag tshuaj xyuas thiab tswj HEPA lim thiab tso pa tawm
- Tsis txhob siv biosafety tub thiab fume hoods interchangeagesen rau cov haujlwm tsis sib xws
- Nco ntsoov ua raws li cov lus qhia PPE tsim nyog: Lab lub tsho tiv no, hnab looj tes, goggles, thiab lwm yam.
Hauv kev xaus
Txawm hais tias ob qho kev nyab xeeb roj ntsha thiab fume hoods yog cov khoom siv tiv thaiv tseem ceeb hauv chaw soj ntsuam, lawv cov lus qhia txog kev sib txawv yog txawv:
-
Nrog rau kev tsom mus rau biosafety, BSC muab kev tiv thaiv tiav rau cov qauv thiab ib puag ncig - zoo tagnrho rau microbiology thiab kev ua haujlwm ntawm tes.
- Fume hoods yog tsim los rau kev nyab xeeb tshuaj lom neeg, tiv thaiv cov neeg ua haujlwm los ntawm cov pa roj teeb muaj kev puas tsuaj thiab cov khoom sib khuam.