Koj puas paub tus qauv tsim qauv ntawm microbiology? Thiab yuav ua li cas kom txuag tau microbes? Nyob rau ntawm qhov no, ib qho me me ntawm cov ntaub ntawv sau tseg thiab sau ua ke. Cia wb mus saib nws nrog Xiaobian ~
Ua ntej, cov ntsiab cai ntawm kev tshawb nrhiav microbiology
Microorganisms muaj cov yam ntxwv ntawm kev sib haum yooj yim. Yog li, nyob rau hauv txoj kev ntawm preservation, cov metabolism hauv microorganisms yuav tsum yog nyob hauv lub xeev tsis ua haujlwm los yog lub xeev li qub, kom lawv tuaj yeem mutated thiab tswj lawv cov peev xwm nyob hauv ib lub sij hawm.
Tsawg kub, txhaws thiab cua cais yog cov tseem ceeb hauv kev txo cov metabolic peev xwm ntawm cov kab mob. Yog li, txawm hais tias muaj ntau txoj kev rau kev txuag ntawm hom, lawv yog cov tsim los raws li peb yam.
Qhov thib ob, txoj kev tiv thaiv tau muab sib faib ua ke rau hauv cov hom nram qab no:
1. Poob haiv thiab kev txuag
Kuj muaj kev sib txuas lus, tus kabmob tawg paj, kabmob kabmob, thiab lwm yam (tom kawg yog siv rau kev txuag cov kab mob hauv anaerobic), thiab muab cia rau hauv lub tub yees thaum 4-6 ° C tom qab cog qoob.
2. Dej ntws paraffin npog pov tseg
Nws yog ib txoj kev tsis sib xws ntawm subculture, uas tuaj yeem ntev sij hawm ntawm kev txuag. Nws yog them nrog cov kua txig ua cov pa tawm ntawm cov kab kos thiab cov kev coj noj coj ua. Ntawm qhov one hand, nws yuav tiv thaiv tau tus kab mob ntawm cov kab mob uas tshwm sim los ntawm kev evaporation ntawm cov kab lis kev cai nruab nrab. Ntawm qhov tod tes, Tiv thaiv cov pa tawm ntawm kev nkag mus rau cov metabolism hauv.
3. Kev tiv thaiv kev tiv thaiv kab mob
Nws yog ib txoj kev uas muab cov kab mob pov tseg rau ntawm ib qho dej nyab, xws li av, xuab zeb, silica gel, lim ntawv, thiab muab ziab ntxiv, xws li cov qauv kev xuab zeb thiab cov ntawv pov tseg.
4. Kev txuag txoj kev tiv thaiv
Rau cov kab mob uas tsis muaj zuj zus hauv cov khoom cua, xws li kab mob viral, rickettsia, spirochetes, thiab lwm yam., Lawv yuav tsum muaj tus kab mob thiab tsiaj txhu hauv cov tsiaj txhu, kab, thiab nqaij qaib embryos. Hom no yog sib npaug ntawm cov kab mob ntawm cov kab mob xwb. Cultivate txoj kev txuag. Microorganisms xws li kab mob vwm kuj tuaj yeem khaws tau los ntawm lwm txoj kev xws li kua nitrogen cia thiab khov thiab ziab kom qhuav.
5. Txoj kev khov nab kuab
Nws tuaj yeem muab faib ua cov thawv txias (-20-30 ° C, -50--80 ° C), cov dej khov qhuav qhuav (txog -70 ° C) thiab kua nitrogen (-196 ° C) thiab lwm yam kev txuag txoj kev.
6. Khov kev ziab kom khov
Cov kab xev yog thawj zaug khov ntawm qhov kub tsis txias (txog -70 ° C), thiab tom qab ntawd ces cov dej raug tshem tawm ntawm qhov tshwm sim hauv kev txo hwjchim (lub tshuab nqus tsev).
Qee txoj kev, xws li cov ntaub ntawv pov tseg, kev siv roj nitrogen cia, thiab txoj kev tiv thaiv kom khov thiab ziab kom qhuav, yuav tsum tau siv tus neeg saib xyuas kev tiv thaiv kom paub txog kev raug tshem tawm ntawm lub cev kom tiv thaiv kev puas hlwb los ntawm kev khov thiab ntub dej sublimation. Cov kev tiv thaiv tiv thaiv kom ruaj ntseg cov txheej txheem ntawm cellular Cheebtsam los ntawm affinity ntawm hydrogen thiab ionic bonds mus rau dej thiab hlwb. Tus neeg saib xyuas kev tiv thaiv muaj xws li nyuj cov kua mis, ntshav dej, qab zib, glycerin, dimethyl sulfoxide thiab cov zoo li.
Koj puas tau pom cov ntsiab lus ntawm Xiaobian nrog kev paub? Yog tias koj xav paub ntau ntxiv txog kev tsim cov qauv tsim, thov koj faj seeb rau peb lub vev xaib website, thiab saib ntsoov rau Xiaobian.